Modeling qRT-PCR dynamics with application to cancer biomarker quantification
نویسندگان
چکیده
منابع مشابه
Development of qRT-PCR Test for Quantification of Rubella Virus in Commercially Available Vaccines
vaccines is considered as a critical point and in-process of quality control (IPQC) test of vaccine production. Rapid tests, like real time PCR, are more appropriated when the production occurs at industrial scale because of the amounts of starting materials and the excess of consumed time required. In the current study, a real-time quantitative reverse transcription-polymerase chain reaction...
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Hantaan virus (HTNV) is a major zoonotic pathogen that causes hemorrhagic fever with renal syndrome (HFRS) in Asia, especially in China. Shaanxi province, which is located in northwest of China, is one of the areas in China most severely afflicted with HFRS epidemics annually. This study aims to establish a quantitative RT-PCR (qRT-PCR) assay to detect HTNV both in cell culture and clinical ser...
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BACKGROUND Management of endometrial carcinoma (EC) still needs improvement of risk assessment. Recently, L1CAM immunohistochemical (IHC) evaluation showed a unique value to predict the outcome of early EC. However IHC results are often conflicting for lack of inter-laboratory standardisation. METHODS Here, as a proof of concept and to increase reproducibility we assayed eighty-two EC and 26 ...
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This study reports on the first quantification of avian influenza virus in the organs of mute swans that died during the epizootic of avian influenza (H5N1) between January and April 2006 in the Czech Republic. The quantitative real-time Reverse Transcriptase PCR (qRT-PCR) assay based on a TaqMan probe was developed for a rapid detection and quantification of avian influenza virus RNA in clinic...
متن کاملEvaluation of bacterial internalization using qRT-PCR.
1Bacterial resistance to antibiotics currently represents a topic of high interest. Unfortunately, studies addressing their adaptation and multidrug tolerance latency are not sufficient for a comprehensive description of the pathogen-host molecular interaction mechanisms (interactome) and the bacterial internalization into human cells, which leads to modified human genes. Bacterial persisters a...
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ژورنال
عنوان ژورنال: Statistical Methods in Medical Research
سال: 2017
ISSN: 0962-2802,1477-0334
DOI: 10.1177/0962280216683204